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MiR-411 functions as a tumor suppressor in renal cell cancer

Abstract

Background

Recent studies have revealed that microRNAs (miRNAs) play important roles as oncogenes or tumor suppressors in tumorigenesis and tumor development, by negatively regulating protein expression. A previous study of microarrays identified that miR-411 was down-regulated in renal cell carcinoma (RCC), while few studies investigating the role of miR-411 in the pathogenesis of RCC have been performed.

Methods

We assessed the miR-411 expression in RCC and paired adjacent normal tissues, as well as in RCC cell lines and a normal renal cell line, by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Furthermore, the effects of miR-411 on RCC and normal renal cell proliferation, apoptosis and migration were determined using MTT assay, CCK-8 assay, flow cytometry and scratch wound assay following restoration of miR-411 with synthetic mimics.

Results

Results of qRT-PCR indicated that the expression of miR-411 was down-regulated in RCC tissues and cell lines when compared with adjacent normal tissues and a normal renal cell line. Further, results of CCK-8, MTT, cell scratch and transwell assay showed that over-expression of miR-411 suppressed RCC cell (786-O and ACHN) proliferation and migration. Flow cytometry assay revealed that miR-411 could induce RCC cell apoptosis. However, overexpression of miR-411 had no obvious effect on normal renal cell line 293T.

Conclusions

To sum up, miR-411 is significantly down-regulated and plays a role as a tumor suppressor in RCC. Further studies are warranted to determine the mechanisms of miR-411 in RCC pathogenesis and define the target genes of miR-411 in RCC.

Int J Biol Markers 2017; 32(4): e454 - e460

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/ijbm.5000261

Authors

Xintao Zhang, Meng Zhang, Jianli Cheng, Zhaojie Lv, Feng Wang, Zhiming Cai

Article History

Disclosures

Financial support: This work was supported by the Shenzhen Basic Research Project (JCYJ20140414170821327).
Conflict of interest: None declared.

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Authors

Affiliations

  • Department of Urology, Shenzhen Second People’s Hospital, Clinical Medicine College of Anhui Medical University, Shenzhen, Guangdong - PR China
  • Graduate School of Anhui Medical University, Hefei, Anhui - P. R. China
  • Department of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong - PR China
  • Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui - PR China
  • Xintao Zhang and Meng Zhang contributed equally to this work.

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