Recent studies have revealed that microRNAs (miRNAs) play important roles as oncogenes or tumor suppressors in tumorigenesis and tumor development, by negatively regulating protein expression. A previous study of microarrays identified that miR-411 was down-regulated in renal cell carcinoma (RCC), while few studies investigating the role of miR-411 in the pathogenesis of RCC have been performed.
We assessed the miR-411 expression in RCC and paired adjacent normal tissues, as well as in RCC cell lines and a normal renal cell line, by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Furthermore, the effects of miR-411 on RCC and normal renal cell proliferation, apoptosis and migration were determined using MTT assay, CCK-8 assay, flow cytometry and scratch wound assay following restoration of miR-411 with synthetic mimics.
Results of qRT-PCR indicated that the expression of miR-411 was down-regulated in RCC tissues and cell lines when compared with adjacent normal tissues and a normal renal cell line. Further, results of CCK-8, MTT, cell scratch and transwell assay showed that over-expression of miR-411 suppressed RCC cell (786-O and ACHN) proliferation and migration. Flow cytometry assay revealed that miR-411 could induce RCC cell apoptosis. However, overexpression of miR-411 had no obvious effect on normal renal cell line 293T.
To sum up, miR-411 is significantly down-regulated and plays a role as a tumor suppressor in RCC. Further studies are warranted to determine the mechanisms of miR-411 in RCC pathogenesis and define the target genes of miR-411 in RCC.
Int J Biol Markers 2017; 32(4): e454 - e460
Article Type: ORIGINAL RESEARCH ARTICLE
AuthorsXintao Zhang, Meng Zhang, Jianli Cheng, Zhaojie Lv, Feng Wang, Zhiming Cai
- • Received on 02/11/2016
- • Accepted on 13/03/2017
- • Available online on 11/07/2017
- • Published online on 31/10/2017
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- Zhang, Xintao [PubMed] [Google Scholar] 1, 2, 3
- Zhang, Meng [PubMed] [Google Scholar] 1, 2, 4
- Cheng, Jianli [PubMed] [Google Scholar] 3
- Lv, Zhaojie [PubMed] [Google Scholar] 3
- Wang, Feng [PubMed] [Google Scholar] 3
- Cai, Zhiming [PubMed] [Google Scholar] 1, 2, 3, * Corresponding Author (email@example.com)
Department of Urology, Shenzhen Second People’s Hospital, Clinical Medicine College of Anhui Medical University, Shenzhen, Guangdong - PR China
Graduate School of Anhui Medical University, Hefei, Anhui - P. R. China
Department of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong - PR China
Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui - PR China
Xintao Zhang and Meng Zhang contributed equally to this work.