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Variations in transrenal DNA and comparison with plasma DNA as a diagnostic marker for colorectal cancer

Abstract

Background

Transrenal DNA can potentially be useful in the disease management of colorectal cancer (CRC) and has potential diagnostic utility. Our study aimed to conduct preoperative and postoperative analysis of KRAS-positive patients, using transrenal DNA compared with plasma DNA. This is critically needed for disease diagnostics and treatment response monitoring.

Methods

CRC patients at different stages of the disease and with different molecular profiles were recruited for serial time-point analysis. Preoperative and postoperative urine specimens were extracted and compared with tumor tissues and plasma DNA.

Results

Our analysis demonstrated that transrenal DNA offered comparable sensitivity and specificity to plasma DNA analysis. Collection of transrenal DNA, being noninvasive, is an attractive assay and easily allows serial monitoring of the disease. Results from preoperative detection showed a close correlation to tumor tissue profiling and demonstrated close associations to the disease. We also observed significant decreases in mutant KRAS DNA concentration after surgery, which confirmed transrenal DNA’s sensitivity to treatment response.

Conclusions

The use of transrenal DNA offers a possible alternative method of disease profiling, detection and tracking. Our study is one of the first to systematically analyze a relatively large number of different CRC patients using transrenal DNA. The positive correlation with disease demonstrates the assay’s viability in the clinical setting, and our method further opens up the possibility to use transrenal DNA for clinical intervention investigations.

Int J Biol Markers 2017; 32(4): e434 - e440

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/ijbm.5000288

Authors

Fang Tian, Yuanfang Liao, Yanfang Zhang

Article History

Disclosures

Financial support: This work was supported by a research grant provided by Jingzhou Central Hospital (201116374).
Conflict of interest: All authors declare they have no conflicts of interest.

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Authors

Affiliations

  • Department of Anaesthesia, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Jingzhou - PR China
  • Department of Laboratory Medicine, Shengli Oilfield Central Hospital, Shandong - PR China

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