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LncRNA GHET1 promotes esophageal squamous cell carcinoma cells proliferation and invasion via induction of EMT

Abstract

Background

Recent studies have shown that long noncoding RNA (lncRNA) gastric carcinoma highly expressed transcript 1 (GHET1) was involved in the progression of tumors. However, the role of GHET1 in esophageal squamous cell carcinoma (ESCC) remains unclear.

Methods

The expression of lncRNA GHET1 was examined in 55 paired ESCC tissues and adjacent nontumor tissues. Molecular and cellular techniques were used to explore the role of GHET1 on ESCC cells.

Results

Our data showed that GHET1 expression was significantly increased in ESCC tissues and cell lines. High GHET1 expression in ESCC tissues was significantly associated with poor differentiation, advanced tumor nodes metastasis stage, and lymph node metastasis. GHET1 showed high sensitivity and specificity for diagnosing ESCC. Our data from in vitro assays showed that GHET1 inhibition suppressed ESCC cells proliferation, migration, and invasion, and induced cells apoptosis. Furthermore, western blot showed that GHET1 inhibition significantly decreased the expression of vimentin and N-cadherin while it increased the expression of E-cadherin.

Conclusions

Our study indicates that GHET1 acts as an oncogene in ESCC and may represent a novel therapeutic target for the treatment of ESCC patients.

Post author correction

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/ijbm.5000304

Authors

Hongfen Liu, Qiang Zhen, Yakun Fan

Article History

Disclosures

Financial support: No grants or funding have been received for this study.
Conflict of interest: None of the authors has financial interests related to this study to disclose.

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Authors

Affiliations

  • Department of Gastroenterology, The First Hospital of Shijiazhuang, Shijiazhuang - PR China
  • Department of Thoracic Surgery, The First Hospital of Shijiazhuang, Shijiazhuang - PR China
  • Department of Geriatrics, The First Hospital of Shijiazhuang, Shijiazhuang - PR China

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