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IQGAP1 silencing suppresses the malignant characteristics of laryngeal squamous cell carcinoma cells

Abstract

Background

Laryngeal squamous cell carcinoma (LSCC) has a poor prognosis due to recurrence and metastasis. IQ-domain GTPase-activating protein 1 (IQGAP1), a scaffold protein, plays an important role in tumorigenesis and malignant development. In this study, we aimed to explore the role of IQGAP1 in LSCC.

Methods

Expression of IQGAP1 in human LSCC specimens was assessed by immunohistochemistry. We also evaluated the roles of IQGAP1 in cell proliferation, migration and invasion and epithelial-to-mesenchymal transition (EMT) in Hep-2 cells.

Results

The expression of IQGAP1 protein was significantly up-regulated in LSCC tissues compared with normal laryngeal tissues (p = 0.002). Furthermore, the knockdown of IQGAP1 in Hep-2 cells inhibited cell growth, migration and invasion. Moreover, we found that IQGAP1 silencing reversed EMT.

Conclusions

These results show for the first time that IQGAP1 is up-regulated in LSCC tissues and plays an important role in LSCC cell proliferation and invasiveness, which indicates that IQGAP1 could work as an oncogene and may serve as a promising molecular target for treatment of LSCC.

Post author correction

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/ijbm.5000287

Authors

Xiaoxia Wang, Chun He, Chaohui Li, Benhong Ren, Qing Deng, Wei Gao, Binquan Wang

Article History

Disclosures

Financial support: This study was supported by grants from the National Natural Science Foundation of China (81372676) and the Postdoctoral Science Foundation of China (2014M551058).
Conflict of interest: The authors declare they have no conflicts of interest.

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Authors

Affiliations

  • Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan - PR China
  • Department of Otolaryngology, Head and Neck tumor key Laboratory of Shanxi Province, Shanxi Medical University, Taiyuan - PR China
  • Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan - PR China

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