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Cullin-1 regulates MG63 cell proliferation and metastasis and is a novel prognostic marker of osteosarcoma

Cullin-1 regulates MG63 cell proliferation and metastasis and is a novel prognostic marker of osteosarcoma

Int J Biol Markers 2017; 32(2): e202 - e209

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jbm.5000247

Authors

Qinghua Cheng, Guoyong Yin

Abstract

Background

There is no reliable marker available for early detection, diagnostic confirmation or disease prognosis of osteosarcoma. Cullin-1 (CUL1) is a newly reported tumor-related gene, and we aimed to unravel its role in osteosarcoma.

Methods

We used immunohistochemistry to analyze the correlation between CUL1 expression and clinicopathological variables and patient survival. To evaluate the function of CUL1, a group of 28 osteosarcoma patients were recruited for this study. The role of regulation of CUL1 in osteosarcoma was studied in vitro and in vivo. In addition, we further investigated the signaling pathway of CUL1 in osteosarcoma progression.

Results

We first discovered that CUL1 expression was up-regulated in human osteosarcoma tissues and inversely correlated with osteosarcoma differentiation. In addition, CUL1 promotes osteosarcoma cell proliferation in vitro and in vivo. We also found that CUL1 promotes osteosarcoma cell invasion and metastasis in vitro and in vivo. High levels of CUL1 promote osteosarcoma progression via up-regulation of MMP9 expression.

Conclusions

Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with osteosarcoma. CUL1 might be an important marker and a therapeutic target for osteosarcoma.

Article History

Disclosures

Financial support: No grants or funding have been received for this study.
Conflict of interest: None.

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Authors

Affiliations

  • Nanjing Medical University, Nanjing - PR China
  • Department of Orthopaedics, People’s Hospital of Lishui District in Nanjing City, Nanjing - PR China
  • Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing - PR China

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