Advertisement

EpCAM-expressing circulating tumor cells in colorectal cancer

Abstract

Background

Several studies have raised the issue of the inadequacy of CellSearch® to detect the entire pool of circulating tumor cells (CTCs) from blood of cancer patients, suggesting that cells expressing low levels of epithelial cell adhesion molecule (EpCAM) are not recognized by the capture reagent. In this exploratory study, we aimed to evaluate the status of EpCAM in CTCs isolated from a group of metastatic colorectal cancer patients, in 40% of whom, CTC had been found to be undetected by the CellSearch® system.

Methods

CTCs were analyzed using both a microfiltration method (ScreenCell) and CellSearch® in parallel. Furthermore, since EpCAM exists in 2 different variants, we investigated the presence of both its intracellular domain (EpICD) and extracellular domain (EpEX) through immunofluorescence staining of CTCs on filters.

Results

Results from immunofluorescence experiments demonstrated that, overall, EpICD and/or EpEX was expressed in 176 CTCs detected by ScreenCell, while the CellSearch® system was able to capture only 10 CTCs.

Conclusions

This is the first demonstration that the low sensitivity of CellSearch® to detect CTCs in colorectal cancer patients is not due to the lack of EpCAM.

Post author correction

Article Type: ORIGINAL RESEARCH ARTICLE

Article Subject: Methods in Biomarkers Research

DOI:10.5301/ijbm.5000284

Authors

Chiara Nicolazzo, Cristina Raimondi, Federica Francescangeli, Simona Ceccarelli, Patrizia Trenta, Valentina Magri, Cinzia Marchese, Ann Zeuner, Angela Gradilone, Paola Gazzaniga

Article History

Disclosures

Financial support: This work was supported by the Italian Ministry for University and Research (MIUR) (grant number C26N147NSX) and A.R.Ger.On. Onlus.
Conflict of interest: The authors have no conflicts of interest to declare.

This article is available as full text PDF.

  • If you are a Subscriber, please log in now.

  • Article price: Eur 36,00
  • You will be granted access to the article for 72 hours and you will be able to download any format (PDF or ePUB). The article will be available in your login area under "My PayPerView". You will need to register a new account (unless you already own an account with this journal), and you will be guided through our online shop. Online purchases are paid by Credit Card through PayPal.
  • If you are not a Subscriber you may:
  • Subscribe to this journal
  • Unlimited access to all our archives, 24 hour a day, every day of the week.

Authors

Affiliations

  •  Circulating Tumor Cells Unit, Department of Molecular Medicine, Sapienza University of Rome, Rome - Italy
  •  Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome - Italy
  •  Department of Experimental Medicine, Sapienza University of Rome, Rome - Italy
  •  Department of Radiological, Oncological and Anatomopathological Sciences, Sapienza University of Rome, Rome - Italy
  • Angela Gradilone and Paola Gazzaniga contributed equally to this work.

Article usage statistics

The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.

No supplementary material is available for this article.