Reliability of urinary 6-sulfatoxymelatonin as a biomarker in breast cancer
Reliability of urinary 6-sulfatoxymelatonin as a biomarker in breast cancer
Int J Biol Markers 2006; 21(4): 242 - 245
Article Type: SHORT COMMUNICATION
Authors
M. Barba, A. Cavalleri, H.J. Schunemann, V. Krogh, A. Evangelista, G. Secreto, A. Micheli, Q. Zhou, B. Fuhrman, B. Teter, F. Berrino, P. Muti
Abstract
The objective of this study is to evaluate the effect of cryopreservation at different storage temperatures on uri-nary 6-sulfatoxymelatonin (aMT6s) concentration. Overnight urine from 28 postmenopausal women participating in the ORDET cohort study was filtered and separated into 6 mL aliquots. Urine samples were stored at -80C and at -30C for an average of 14 years. Urinary aMT6s concentration was assessed using a competitive immunoassay. Mean aMT6s values of samples stored at -30C were systematically lower than those of samples stored at -80C (10.7 ng/mL versus 15.8 ng/mL, p<0.001). Bland Altman plots showed disagreement between determinations at different storage temperatures at the high-est levels of the metabolite concentration. The degree of agreement evaluated in terms of intra-class correlation coefficient was 0.68 (95% CI 0.41-0.84, p<0.0001). Pearsons correlation coefficient between aMT6s values of the two differently stored samples was 0.93 (p<0.001), while the Kendal tau coefficient for rank distribution was 0.73 (p<0.001). Our data suggest that storage temperatures might affect degradation of aMT6s during storage. However, individual characterization by melatonin levels does not seem to be affected by cryopreservation conditions.
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Authors
- Barba, M.
[PubMed]
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Department of Epidemiology, Regina Elena Cancer Institute, Rome and Department of Endocrinology and
- Cavalleri, A.
[PubMed]
[Google Scholar]
Department of Preventive and Predictive Medicine, National Cancer Institute, Milan - Italy
- Schunemann, H.J.
[PubMed]
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Department of Epidemiology, Regina Elena Cancer Institute, Rome - Italy and Department of Clinical E
- Krogh, V.
[PubMed]
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Department of Preventive and Predictive Medicine, National Cancer Institute, Milan - Italy
- Evangelista, A.
[PubMed]
[Google Scholar]
Department of Preventive and Predictive Medicine, National Cancer Institute, Milan - Italy
- Secreto, G.
[PubMed]
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Department of Preventive and Predictive Medicine, National Cancer Institute, Milan - Italy
- Micheli, A.
[PubMed]
[Google Scholar]
Department of Preventive and Predictive Medicine, National Cancer Institute, Milan - Italy
- Zhou, Q.
[PubMed]
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Department of Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, ON - Canada
- Fuhrman, B.
[PubMed]
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Department of Social and Preventive Medicine, University at Buffalo, State University of New York, B
- Teter, B.
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[Google Scholar]
Department of Social and Preventive Medicine, University at Buffalo, State University of New York, B
- Berrino, F.
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Unit of Epidemiology, Department of Preventive Medicine, National Cancer Institute, Milan - Italy
- Muti, P.
[PubMed]
[Google Scholar]
Department of Epidemiology, Regina Elena Cancer Institute, Rome - Italy